Purpose: We are doing this experiment to see first hand how bacteria reproduces and how fast they can grow and develop when placed in ideal living conditions.
Hypothesis: If we gather bacteria in an agar dish and place in ideal growth and development conditions (incubator) than the bacteria that we collect will reproduce and increase in size because they are given the opportunity to grown and develop. Material: 3 Cotton Swabs, Agar Dish, Incubator, Sterilizing Inoculating Loop, dH2O, Clean Slide, Bunson Burner, Crystal Violet Stain, Gram's Iodine, 95% Ethanol, Safrinin, Blot Dry Cloths, Disinfectant Agent Method: 1) Go around school and swab four different surfaces with two double sided cotton swabs. 2) Grab an agar dish and label off four quadrants named A B C D. 3) Cover each section with the bacteria that matches the quadrant ie) locker bacteria goes to quad A. 4) Seal the lid on and place in the incubator. 5) Come back a couple days later and examine how much your bacteria has grown and reproduced. 6) Examine your bacteria and take note of how far it has come (size, colour, shape, colony size, etc.) 7) Sterilize inoculating loop. 8) Add one drop of dH2O onto a clean slide. 9) Make smear of bacteria onto the clean slide (make sure it is just bacteria and not agar) and re-sterilize the inoculating loop. 10) Gently dry the slide : hold slide well above the flame and pass it through the flame until dH20 is evaporated... you should be able to tough and hold the slide. 11) Next up is the Primary Stain, first apple Crystal Violet stain for 1-2 minutes and then rinse with dH20. 12) Apply Gram's Iodine for 1-2 minutes and then rinse with dH20. 13) Wash with 95% ethanol until the purple stops washing away, then rinse with dH20 right away. 14) Counter Stain: Apply Sanfrinin for 1-2 mintues, rinse with dH2O and blot dry. 15) Clean up: Disinfect your work area. Results: |
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